The specific aims of the project are to understand how oscillatory gene expression is generated and its functional importance of cell state transitions. We will interrogate the relative contribution of transcription and translation, using live imaging at single cell level to visualise mRNA production, mRNA translation and protein dynamics. Parameters governing these processes will be quantitated and used to inform realistic computational models, in collaboration with theoreticians in the lab. To understand the functional importance we will develop and implement methods to manipulate oscillations.
We are recruiting an ambitious, self motivated and experimentally talented post-doc to join us in understanding the regulation and role of biological oscillations in cell state transitions.
The post is funded by the Wellcome Trust and is available from 1st of September 2022, with some flexibility, on a full-time basis, fixed term for three years. Candidates must already hold a PhD or expect to shortly in a Biological discipline and have at least one first author publication. The successful candidate will have theoretical and practical knowledge in developmental, cancer or stem cell biology, excellent technical skills in molecular biology and experience with confocal live imaging of cells. Experience with scRNAseq, bioinformatics skills, coding ability and prior experience working in an interdisciplinary team would be an advantage.
Essential Skills, Knowledge and Experience
- Hold (or expect to hold shortly) a PhD in a Biological Discipline.
- Have at least one first author research publication in the last four years in an International Journal.
- Extensive and up-to-date theoretical and practical knowledge in Developmental, Cancer or Stem Cell Biology.
- Excellent technical skills in Molecular Biology.
- Extensive experience in Confocal Microscopy, Molecular Imaging at the Single Cell Level (live and fixed, i.e. smFISH) and Image analysis.
- Extensive experience in tissue culture and nucleofection, including generating CRISPR/Cas9 fluorescent Knock-in reporter clonal lines.
- Solid grounding in Statistics.
- Proven ability to use initiative to efficiently plan, optimise and progress project and communicate findings.
- Excellent interpersonal and communication skills and ability to work with colleagues at all levels.
- Good written and spoken English.
- A willingness to contribute to the work of others by offering practical and intellectual help.
- Strong collaborative ethos.
The School/Department is strongly committed to promoting equality and diversity, including the Athena SWAN charter for gender equality in higher education. The School/Department holds a Silver Award which recognizes their good practice in relation to gender; including flexible working arrangements, family-friendly policies, and support to allow staff achieve a good work-life balance. We particularly welcome applications from women for this post. All appointment will be made on merit. For further information, please visit:
Our University is positive about flexible working – you can find out more here
Blended working arrangements may be considered
Please note that we are unable to respond to enquiries, accept CVs or applications from Recruitment Agencies.
Enquiries about the vacancy, shortlisting and interviews:
Manager: Professor Nancy Papalopulu
This vacancy will close for applications at midnight on the closing date.
Please see the link below for the Further Particulars document which contains the person specification criteria.