Spontaneous mutation is a fundamental biological process that drives evolutionary innovations and generates the key global challenge of antimicrobial resistance. What we need is a deep understanding of mutation dynamics in a single bacterial cell growing in its native environment, a community.
The aim of this project is to combine a live fluorescence microscopy and microfluidics to measure the molecular processes involved in mutation avoidance and DNA repair. You will track single molecules of Nudix hydrolases and DNA repair proteins, and determine roles of environments and cell-cell interactions on the dynamics of these proteins. The project is based on the previous work showing that cell density and cell-cell interactions are critical for mutation rates in microbial batch cultures (Krašovec et al., Nature Commun., http://doi.org/skb; Krašovec et al., Plos Biology, http://doi.org/cb9s).
This inter-disciplinary project will deepen our fundamental understanding of how evolution works and knowledge generated in this project will enable us to better predict mutation-based resistance in microbial communities. This could extend the usefulness of existing antibiotics and inform the development of longer-lasting novel drugs.
This is a 3-year full-time position in the first instance aiming to start in November 2020 (negotiable).
The ideal candidate is an outstanding experimentalist who holds (or expects to hold shortly) a PhD in microbial evolution, microbial ecology, molecular biology, biochemistry, biophysics, biophysical chemistry or related field.
You should have extensive and up-to-date theoretical and practical knowledge either in live fluorescence microscopy/quantitative cell imaging or in molecular biology/DNA repair/protein-nucleic acid interactions. Being experienced in both areas is an advantage but it is not necessary, because we will provide appropriate training, substantial technical support and expertise for this position. However, enthusiasm for the project and a strong interest in crossing research fields are essential.
You will work together with a full-time research technician exclusively associated with this UKRI funded research programme, with the aim to study factors that affect cells’ capability to avoid and repair mutations.
The integrated structure of our Faculty enables a truly translational approach to biology, medicine and health - from pure discovery science through to clinical application and patient care. It also encourages collaborative working, enabling staff to deliver innovative, world-leading research that has a very real and positive impact on people’s lives, as well as high-quality education and training to over 11,000 undergraduate and postgraduate students.
The School is strongly committed to promoting equality and diversity, including the Athena SWAN charter for gender equality in higher education. The School holds a Silver Award which recognises their good practice in relation to gender; including flexible working arrangements, family-friendly policies, and support to allow staff achieve a good work-life balance. We particularly welcome applications from women for this post. All appointment will be made on merit. For further information, please visit:
Please note that we are unable to respond to enquiries, accept CVs or applications from Recruitment Agencies.
Enquiries about the vacancy, shortlisting and interviews:
Name: Rok Krašovec
This vacancy will close for applications at midnight on the closing date.
Please see the link below for the Further Particulars document which contains the person specification criteria.